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nsdv gn  (Native Antigen Inc)


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    Native Antigen Inc nsdv gn
    Production and characterization of recombinant <t>NSDV</t> GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains <t>(Ganjam</t> <t>virus</t> IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.
    Nsdv Gn, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsdv+gn/Nairobi+Sheep+Disease+Virus+Glycoprotein+Gn%2C+Human+Fc-Tag+(HEK293)/pmc12282141-201-0-12
    Average 94 stars, based on 2 article reviews
    nsdv gn - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing"

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing

    Journal: Journal of Virology

    doi: 10.1128/jvi.00537-25

    Production and characterization of recombinant NSDV GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains (Ganjam virus IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.
    Figure Legend Snippet: Production and characterization of recombinant NSDV GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains (Ganjam virus IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.

    Techniques Used: Recombinant, Virus, Glycoproteomics, Protein Purification, Sequencing, FLAG-tag, SDS Page, Purification, Staining, Western Blot

    Related Articles

    Concentration Assay:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Recombinant:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Virus:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Glycoproteomics:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Protein Purification:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Sequencing:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    FLAG-tag:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    SDS Page:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Purification:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Staining:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.

    Western Blot:

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing
    Article Snippet: After three washes with PBST, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate (Bio-Rad, Munich) was added.



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    Production and characterization of recombinant <t>NSDV</t> GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains <t>(Ganjam</t> <t>virus</t> IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.
    Nsdv Gn, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsdv+gn/Nairobi+Sheep+Disease+Virus+Glycoprotein+Gn%2C+Human+Fc-Tag+(HEK293)/pmc12282141-201-0-12
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    Production and characterization of recombinant <t>NSDV</t> GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains <t>(Ganjam</t> <t>virus</t> IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.
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    Image Search Results


    Production and characterization of recombinant NSDV GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains (Ganjam virus IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.

    Journal: Journal of Virology

    Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing

    doi: 10.1128/jvi.00537-25

    Figure Lengend Snippet: Production and characterization of recombinant NSDV GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains (Ganjam virus IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.

    Article Snippet: NSDV Gn ( NAC-REC31904-500 ) and NSDV Gc ( NAC-REC31906-500 ) from The Native Antigen Company were diluted in 0.01 M PBS and coated overnight on Maxisorp 96-well plates at a concentration of 100 ng/well.

    Techniques: Recombinant, Virus, Glycoproteomics, Protein Purification, Sequencing, FLAG-tag, SDS Page, Purification, Staining, Western Blot